Authors
William C. Skarnes & Xingxu Huang
Summary
Cas9 nickase and paired sgRNAs can be used to efficiently modify the genome of one-cell embryos with minimal off-target damage1. This protocol describes the design and construction of paired sgRNAs plasmids, preparation of sgRNAs and Cas9-D10A mRNAs for the injection of one-cell embryos, and, genotyping of founder animals.Further details
The protocol was published on Protocol Exchange on 3 March 2014. To see the entire protocol, click on the source link.Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 678
- Comments 0