Authors
Romain Legrand, Kuniko Takagi & Sergueï Fetissov
Summary
Circulating peptide hormones such as ghrelin physiologically bind plasma immunoglobulins (Ig) which protect hormone from degradation and modulate its biological activity depending on affinity of hormone / IgG binding. Because the IgG set of each individual is unique, measuring affinity kinetics of human or animal plasma IgG binding to a specific peptide hormone may provide useful information towards understanding of individual variability in peptidergic signaling. Here, we describe a protocol of IgG preparation from human blood samples adapted for the analysis of affinity kinetics binding between IgG and peptide hormones measured by the surface plasmon resonance. The same protocol can be also applied to analyze IgG in rodents.Further details
The protocol was published on Protocol Exchange on 26 February 2014. To see the entire protocol, click on the source link.Advertisement
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