Authors
Laurent Dembélé, Jean-François Franetich, Audrey Lorthiois, Audrey Gego, Georges Snounou & Dominique Mazier
Summary
The dormant liver stages, hypnozoites, of some malaria parasite species were not amenable to in vitro studies because primary hepatocyte cultures could not be maintained beyond 10-14 days. In this protocol we describe a co-culture system where primary simian hepatocyte could be maintained for a month or more through co-cultivation with an immortalised liver derived cell line, Hepa-RG. The hepatocytes can be infected with Plasmodium sporozoites and the persisting hypnozoites can then observed and investigated.Further details
The protocol was published on Protocol Exchange on 11 February 2014. To see the entire protocol, click on the source link.Advertisement
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