Authors
Pradip Nahar
Summary
In this protocol, we describe a unique ELISA method for the detection of an antigen or antibody, in which ELISA steps are carried out by pressure incubation instead of conventional thermal incubation. Pressure-mediated ELISA (PELISA), carried out in 1 h shows more than a 2-fold increase in absorbance value than the control experiment carried out at the same time and temperature without applying pressure. Estimation of analyte by the 1-h PELISA method gives similar absorbance value to that obtained by 3-h heat-mediated ELISA (HELISA) on an activated surface. As PELISA is sensitive, specific, and reproducible, it could be an excellent alternative to HELISA or conventional ELISA procedure.Further details
The protocol was published on Protocol Exchange on 10 December 2013. To see the entire protocol, click on the source link.Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 278
- Comments 0