Authors
Yuwei Yao, Xiaoli Sun, Xiaotian Cui & Boxun Lu
Summary
Human stem cell (SC)-derived neurons provide excellent cellular models for human neurodegenerative disorders; unfortunately, there is a lack of protocols to perform screenings in such models. We have recently developed a high-throughput high-content assay that potentially enables such screenings in SC-derived Huntington’s disease (HD) neurons. To ensure the screening feasibility, we have run a small-scale screen for modifiers of HD toxicity with this protocol and confirmed the screening quality. The assay provides high-throughput high-content imaging data, quantifies neurodegeneration directly in the incubator, and can easily be executed by a graduate student level scientist; showing advantages over existing neurodegeneration screening assays at least in certain aspects. The assay protocol allows potential screenings for HD and other neurodegenerative disorders, and thus provides a valuable tool for both basic and translational research in the neurodegeneration field.Further details
The protocol was published on Protocol Exchange on 22 November 2013. To see the entire protocol, click on the source link.Advertisement
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