Authors
Shin-Ichi Mae & Kenji Osafune
Summary
The induction of kidney lineage cells from human pluripotent stem cells (hPSCs) has been previously described in some reports, but there have been no protocols so far for the robust induction of intermediate mesoderm (IM), an early embryonic germ layer that gives rise to kidneys. In the present study, we have established a robust induction protocol using combinational treatment of growth factors, which produces up to 90% IM cells expressing the IM-specific marker OSR1. This novel method thus supplies an unprecedented system to elucidate the mechanisms of IM development and potentially provide a cell source for kidney regeneration.Further details
The protocol was published on Protocol Exchange on 18 September 2013. To see the entire protocol, click on the source link.Advertisement
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