Authors
Edward Farmer, Seyed Mousavi, and Aurore Lenglet
Summary
Two of the major features of long distance signals are where they travel and how fast this occurs. The spatial analysis of long distance signals is often necessary prior to temporal analysis. Spatiotemporal analysis lays the foundations for discovering signal propagation mechanisms. This protocol concerns spatial aspects of long distance signalling in Arabidopsis rosettes and uses wounding as the example. However, the principle is applicable to other plants and to other stimuli (pathogenesis, localised high light stress or localised temperature changes etc.). The key to spatial analysis of long distance signalling rests on using uniform populations of plants and giving every relevant organ (e.g. leaf) its own identity prior to analysis. Doing this greatly enhances the reproducibility of experiments and allows researchers to examine questions such: Are source-sink pathways used to conduct long distance signals? or, Are long distance signals bidirectional, travelling from young to old leaves and vice versa ? By carefully numbering each leaf it is possible to investigate signalling within and outside domains formed by interleaf vascular connections. Leaf-to-leaf vascular connections (parastichies) in Arabidopsis rosettes have been delineated 1.Further details
The protocol was published on Protocol Exchange on 27 August 2013. To see the entire protocol, click on the source link.Advertisement
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