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A Protocol to Retain Weakly Interacting Protein Complexes for Structural Studies using an Appropriate Linker

External protocol Created on 30 Apr 2014

Authors

Vishnu Priyanka Reddy Chichili, Veerendra Kumar, and J. Sivaraman

Summary

This article describes a protocol to trap protein complexes for structural studies using an appropriate linker to fuse the interacting partners. We have employed this method to trap transiently interacting protein complexes in which one of the binding partners is unstructured.

Further details

The protocol was published on Protocol Exchange on 27 June 2013. To see the entire protocol, click on the source link.

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