Authors
Dario Besusso, Mirjam Geibel, and Liliana Minichiello
Summary
Huntington’s disease (HD) is a genetic neurological disorder characterized by early degeneration of enkephalin-expressing striatal neurons, also known as Medium-sized Spiny Neurons (MSNs). Therefore, accurate immunodetection of enkephalin expressing cells is of wide interest for the scientific community. Here, we describe an immunohistochemical procedure for the detection of the unprocessed precursor of the opioid peptide met-enkephalin, namely the preproenkephalin (Penk1), in mouse brain cryosections. The specificity of the immunolabeling has been confirmed by crossing a reporter mouse line whereby EYFP expression is obtained upon Cre-mediated recombination in animals carrying Cre recombinase under the control of the preproenkephalin promoter.Further details
The protocol was published on Protocol Exchange on 18 June 2013. To see the entire protocol, click on the source link.Advertisement
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