Authors
Ming-Ming Li*, Yue-He Ding*, Anja Nilsen, Arne Klungland, Meng-Qiu Dong, and Yun-Gui Yang
Summary
Proteins can be methylated on the side-chain nitrogens of lysine and arginine residues or on carboxy-termini. Protein methylation is a way of subtly changing the primary sequence of a peptide so that it can encode more information. This common posttranslational modification is implicated in the regulation of a variety of processes including protein trafficking, transcription and protein-protein interactions. In this protocol, we will describe how to use the mass spectrum to verify actin K84 monomethylation.Further details
The protocol was published on Protocol Exchange on 22 May 2013. To see the entire protocol, click on the source link.Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 343
- Comments 0