Authors
Sundari Chetty, Felicia Walton Pagliuca, Christian Honore, and Douglas A. Melton
Summary
We describe a method to help overcome restrictions on the differentiation propensities of human pluripotent stem cells. Culturing pluripotent stem cells in dimethylsulfoxide (DMSO) activates the retinoblastoma protein, increases the proportion of cells in the early G1 phase of the cell cycle and, in more than 25 embryonic and induced pluripotent stem cell lines, improves directed differentiation into multiple lineages. DMSO treatment also improves differentiation into terminal cell types in several cell lines.Further details
The protocol was published on Protocol Exchange on 13 May 2013. To see the entire protocol, click on the source link.Advertisement
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