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Generation of TetOff-inducible Ago2 stable clones for studying miRNA processing in vivo

External protocol Created on 30 Apr 2014

Authors

Mien- Chie Hung and Jia Shen

Summary

Here we describe a method for studying WT or Mutant Ago2-mediated miRNA maturation in vivo through generation of TetOff-inducible Ago2 stable clones expressing WT or Mutant pri-miRNAs.

Further details

The protocol was published on Protocol Exchange on 3 May 2013. To see the entire protocol, click on the source link.

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