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Protocol for lentiviral knock down in mouse ES cells

External protocol Created on 30 Apr 2014

Authors

W.W.M. Pim Pijnappel, Marijke P.A. Baltissen, and H.T.Marc Timmers

Summary

This protocol describes the procedure for lentiviral-mediated knock down of transcription factors like TFIID in mouse embryonic stem cells, and how this can be used to test for factors that are required to maintain the pluripotent state.

Further details

The protocol was published on Protocol Exchange on 26 March 2013. To see the entire protocol, click on the source link.

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