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Reprogramming efficiency of human embryonic fibroblasts using polycistronic lentiviral expression of reprogramming factors OSKM. Testing of factors that enhance efficiency

External protocol Created on 30 Apr 2014

Authors

W.W.M. Pim Pijnappel, Erik van der Wal, Holm Zaehres, Hans. R. Schöler, and H.T.Marc Timmers

Summary

This protocol describes the testing of gene products for enhancement of reprogramming to iPS cells from human embryonic fibroblasts. OSKM factors together with the gene of interest are expressed uisng lentiviruses.

Further details

The protocol was published on Protocol Exchange on 19 March 2013. To see the entire protocol, click on the source link.

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