Authors
Liangzhong Xiang and Huabei Jiang
Summary
Four dimensional (4D) photoacoustic tomography (PAT) has recently been developed to offer dynamic three-dimensional (3D) structural and functional imaging of living biological tissue with label-free, optical absorption contrast. 4D PAT integrates time resolution with three dimensional (3D) spatial resolution by using spherical arrays of ultrasonic detectors. The technique generates motion pictures of imaged tissue, enabling real time tracking of dynamic physiological and pathological processes at hundred micrometer-millisecond resolutions. Here we provide a detailed description of the 4D PAT imaging system setup, signal processing and data acquisition, system calibration, image reconstruction and animal handling. It currently takes ~300 ms for data acquisition by using a 10-Hz pulse -repetition rate laser system. The data acquisition time, however, can be significantly reduced by using a laser system with a higher pulse repetition rate.Further details
The protocol was published on Protocol Exchange on 18 March 2013. To see the entire protocol, click on the source link.Advertisement
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