Authors
Fang Ni, Rui Sun, Binqing Fu, Fuyan Wang, Chuang Guo, Zhigang Tian, and Haiming Wei
Summary
A number of different cytokine combinations have been tested for their ability to support the proliferation and differentiation of human CD34+ hematopoietic stem cells (HSC) into mature NK cells. Here, we describe an improved protocol based on multiple cytokines (SCF, Flt3-L, IL-15 and IGF-1) culture for obtaining a high number of lytic NK cells from human umbilical cord blood CD34+ cells.More than 6×107 highly lytic NK cells can be obtained from 1×105 fresh CD34+ HSC using this protocol. This facilitates the use of the above culture system as a tool for researchers to study the development, properties and functions of NK cells in vitro, and this procedure also facilitates cellular adoptive immunotherapy by providing sufficient numbers of highly enriched CD3- CD56+ effector cells with highly cytotoxic potential.Further details
The protocol was published on Protocol Exchange on 18 February 2013. To see the entire protocol, click on the source link.Advertisement
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