Authors
Shyam Patel, Sharon Pine, and Pranela Rameshwar
Summary
Time-lapse video microscopy is a technique that allows one to assess cellular behavior in real-time. Two-dimensional image data is repeatedly collected at distinct time intervals, and a movie is assembled. Applications include assessment of cell migration, division, communication, growth, and death. A unique advantage to time-lapse video microscopy for assessment of these parameters is that data is collected at the single-cell level, allowing one to assess heterogeneity within a mixed population of cells. Therefore, this is particularly advantageous for cancer biology and stem cell biology. In this protocol, we describe the use of time-lapse video microscopy for (1) the real-time monitoring of cell cycle kinetics of heterogeneous populations of cancer cells, and (2) the real-time assessment of symmetric versus asymmetric stem cell division.Further details
The protocol was published on Protocol Exchange on 11 February 2013. To see the entire protocol, click on the source link.Advertisement
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