Authors
Shyam Patel, Sharon Pine, and Pranela Rameshwar
Summary
The noble agar assay is based on the principle that the cancer cells are anchorage-independent and do not show contact inhibition. This type of soft agar assay can give insight into two important properties of a subset of cancer cells. Firstly, the tumorigenic efficiency of cancer cells can be determined based on number of colonies that emerge. This is based on the ability of cells to grow unattached and to remain suspended in agar. Secondly, the self-renewal properties of cancer cells can be assessed based on the types of progeny produced in noble agar.Further details
The protocol was published on Protocol Exchange on 11 February 2013. To see the entire protocol, click on the source link.Advertisement
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