Authors
Takayuki Uozumi, Ryuji Yamada, Akiya Suzuki, Gun Taniguchi, Kazushi Yoshida, Yuichi Iino, Takeshi Ishihara, and Takaaki Hirotsu
Summary
Signal transduction pathways play essential roles to adapt environmental changes and composed of various signaling proteins. Therefore, observation of activity of a specific protein in vivo is important for understanding the signal transduction pathways. However temporal dynamics of signaling proteins in living animals are not well known. Here we tried to monitor the activities of Ras protein which is one of the key regulators for the Ras-MAPK signal transduction pathway. It was previously reported that the Ras-MAPK pathway functions in olfactory neurons in Caenorhabditis elegans. Thus, we observed Ras activity in olfactory neuron in living animals using Raichu-Ras, a FRET-based sensor for Ras activity. We succeeded in detecting quick changes of Ras activity in response to odor stimuli.Further details
The protocol was published on Protocol Exchange on 6 February 2013. To see the entire protocol, click on the source link.Advertisement
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