Authors
Marco Ranzani, Stefano Annunziato, Fabrizio Benedicenti, Pierangela Gallina, Luigi Naldini, and Eugenio Montini
Summary
Linear Amplification Mediated (LAM) PCR is a method to retrieve the integration sites of different integrating vectors into the genome1-3. A PCR product that starts from the known sequence of the vector and extends through the unknown flanking genome is generated and sequenced to identify the position within the genome of the vector integration. Here we describe the adaption and exploitation of LAM-PCR for the retrieval of lentiviral vector (LV) integrations from oligoclonal hepatocellular carcinomas induced by LV-based insertional mutagenesis4.Further details
The protocol was published on Protocol Exchange on 30 January 2013. To see the entire protocol, click on the source link.Advertisement
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