Authors
Vanessa Sperandio
Summary
Autophosphorylation activity of histidine-kinases (HK) reconstituted into liposomes has been successfully employed in the Sperandio laboratory to study the response of HKs to chemical cues (1, 2). One of the challenges of performing autophosphorylation assays with membrane-bound HKs is the purification of functional proteins for liposome loading and reconstitution. In this protocol, we describe the liposome reconstitution of the multi-transmembrane domain HK FusK of Enterohemorrhagic Escherichia coli (EHEC) O157:H7, and in vitro autophosphorylation activity in response to L-fucose (5).Further details
The protocol was published on Protocol Exchange on 27 November 2012. To see the entire protocol, click on the source link.Advertisement
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