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Injection of Retrograde Beads into the Nucleus Accumbens (NAc) and Medial Prefronral Cortex (mPFC) to Isolate Projection-Specific Neurons in the Ventral Tegmental Area (VTA)

External protocol Created on 30 Apr 2014

Authors

Jessica Walsh*, Allyson Friedman*, Dipesh Chaudhury*, Barbara Juarez, Stacy Ku, and Ming-Hu Han

Summary

Retrograde dyes, such as lumafluors, have been used as tracers to visualize neurons that project to a specific target region. Injection of these dyes provides an important method in being able to understand the functional role of projection-specific neurons. Lumafluors can be directly injected into a target brain region of a mouse and dye positive cells from the projection region can be electrophysiologically recorded in order to understand their projection-specific functions.

Further details

The protocol was published on Protocol Exchange on 10 October 2012. To see the entire protocol, click on the source link.

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