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Optogenetic manipulation of Ventral Tegmental Area (VTA) Neurons that project to the Nucleus Accumbens (NAc) and medial Prefrontal Cortex (mPFC)

External protocol Created on 30 Apr 2014

Authors

Jessica Walsh*, Dipesh Chaudhury*, Allyson Friedman, Barbara Juarez, Stacy Ku, Mary Kay Lobo, and Ming-Hu Han

Summary

Optogenetics has evolved to be a critical technique used to manipulate the firing activity of specific subsets of neurons. Through the use of specific firing parameters, it has become possible to control the behavior of freely moving animals. Here we have established a system to control projection pathway-specific neurons from a particular brain region through the combined use of a Cre-dependent ChR2 and a transceullar, retrograde, Cre virus.

Further details

The protocol was published on Protocol Exchange on 10 October 2012. To see the entire protocol, click on the source link.

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