Authors
Michelle Lee, Florian Muller, Elisa Aquilanti, Baoli Hu, and Ronald DePinho
Summary
Xenografting of human cancer cell lines injected subcutanously in nude mice has become the standard testing platform for dissecting mechanistic aspects of tumorigenesis and for pre-clinical drug development. However, subcutanous xenografts do not model tumors in their tissue of origin and thus may have some clinically relevant limitations. Xenografted human tumor cells directly injected into their relevant organ may present a more accurate model of human tumors. Here, we describe a rapid protocol for the injection of tumor cell lines into the mouse brain for the establishment of orthotopic tumors.Further details
The protocol was published on Protocol Exchange on 22 August 2012. To see the entire protocol, click on the source link.Advertisement
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