Authors
Caroline Uebel, Sonja Koch, Anja Maier, Nina Sopel, Anna Graser, Stephanie Mousset, and Susetta Finotto
Summary
Innovative local immunotherapy for severe lung diseases such as asthma, chronic obstructive pulmonary disease or lung cancer requires a successful delivery to access the desired cellular target in the lung. An important route is the direct instillation into the airways in contrast to delivery through the digestive tract. This protocol details a method to deliver antibodies, recombinant cytokines, small inhibitory molecules and other molecular therapeutics to induce an effective protective immune response in the airways. The therapeutic is delivered under anaesthetic by dropping it onto the nostrils of the mouse, so that the animal can inhale it. It is possible to complete the whole procedure within 5 minutes. To ensure a correct delivery of the substance into the lung, it can be labelled with fluorescent dyes and monitored in the lung later on. Additionally, fluorescent labelled antigen can be applied and the target cell might be visualized after antigen up-take in vivo.Further details
The protocol was published on Protocol Exchange on 10 July 2012. To see the entire protocol, click on the source link.Advertisement
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