Authors
James Cronk, Noel Derecki, and Jonathan Kipnis
Summary
This protocol describes how to measure neuron soma size by fluorescent Nissl stain. Mice are sacrificed, and fixed by PFA perfusion. Brains are removed, and further PFA fixed, followed by sucrose cryoprotection. They are then snap frozen, sliced by cryostat, and stained with fluorescent Nissl as floating sections. Confocal microscopy is used to take images of neurons, and a computer graphics tablet is used to calculate individual neuron soma sizes.Further details
The protocol was published on Protocol Exchange on 6 June 2012. To see the entire protocol, click on the source link.Advertisement
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