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Probabilistic mapping and image clustering for quantitative assessment of fluorescent protein localizations in Arabidopsis guard cells

External protocol Created on 30 Apr 2014

Authors

Takumi Higaki, Natsumaro Kutsuna, and Seiichiro Hasezawa

Summary

The protocol reported here describes a method to quantitatively evaluate fluorescently-tagged protein localizations from fluorescent microscopic images with a combination of probabilistic mapping and image clustering. We demonstrate the use of this protocol using kidney-shaped guard cells of plants.

Further details

The protocol was published on Protocol Exchange on 21 May 2012. To see the entire protocol, click on the source link.

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