Authors
Hamid Kalantari, Hamid Gourabi, and Hossein Baharvand
Summary
Our observation showed the addition of cytochalasin-B to mouse embryonic stem cells (mESC) culture for CBMn analysis led to the induction of apoptosis in these cells. On the other hand, addition of cyt-B is the most critical part of the cytokinesis-block micronucleus assay (CBMn) technique that cannot be omitted. Thus, modification of the traditional CBMn assay seems to be necessary. In this paper, we attempt to troubleshoot this problem and show that the CBMn assay can be used in embryonic stem cells research, particularly as a reliable tool in monitoring the cytogenetic integrity of cells. The potential reasons of the observed induction of apoptosis in mESCs are also discussed.Further details
The protocol was published on Protocol Exchange on 12 April 2012. To see the entire protocol, click on the source link.Advertisement
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