Authors
Palanimurugan R and R Jürgen Dohmen
Summary
We have established an ultrafiltration-based in vitro assay for determining polyamine binding to proteins. In the protocol described here we have used Saccharomyces cerevisiae ODC antizyme protein produced in E. coli and radioactive polyamines to measure the direct binding of polyamines to ODC antizyme protein.Further details
The protocol was published on Protocol Exchange on 29 February 2012. To see the entire protocol, click on the source link.Advertisement
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