Authors
Jennifer Gommerman and Olga Rojas
Summary
We report that IgA+ PC also can produce the anti-microbial mediators TNFα and iNOS which appears to arise in the unique environment of the gut, and may be critical to mount effective responses to microbial assault. To examine the functional relevance of TNFα/iNOS expression in IgA+ cells, we created mixed-bone marrow (BM) chimeras from TNFα/iNOS double knockout mice (dKO), in combination with B-cell deficient (JH-/-), using JH-/- or Rag2-/- mice lethally irradiated recipients, thus creating mixed BM chimeras in which B cells are unable to produce TNFα/iNOS. Here we describe in detail the generation of these mixed bone marrow chimeras.Further details
The protocol was published on Protocol Exchange on 16 December 2011. To see the entire protocol, click on the source link.Advertisement
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