Authors
Yinglang Wan, Eric McLamore, Lusheng Fan, Huaiqing Hao, D. Marshall Porterfield, Zengkai Zhang, Wenjun Wang, Yue (Jeff) Xu, and Jinxing Lin
Summary
This protocol describes an integration of the Non-invasive Micro-test Technique and Oxygen Optrode (NMT-OO) to quantify rhizosphere oxygen fluxes in Arabidopsis. The optrode has high sensitivity and selectivity in the measurement of oxygen concentrations and fluxes at the cellular level. In particular, application of the NMT-OO using the self-referencing method avoids environmental electromagnetic noise and hysteresis/calibration drift, providing extremely high signal-to-noise ratios for measuring biophysical transport. We successfully applied this technique to measure rhizosphere oxygen fluxes and metabolism in intact roots of Arabidopsis plants. The system we describe here is a simple and reliable method for measuring oxygen fluxes in plants and has other broad applications in cytological studies.Further details
The protocol was published on Protocol Exchange on 10 November 2011. To see the entire protocol, click on the source link.Advertisement
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