Authors
Roger J. Hajjar, Changwon Kho, Ahyoung Lee, Dongtak Jeong, Jae Gyun Oh, Antoine H. Chaanine, Jiqiu Chen, Eddy Kizana, and Woo Jin Park
Summary
Cardiac gene transfer represents a unique strategy to alter the expression of specific genes in the heart that may result in alterations in contractile function. This approach allows us to investigate the role of these pathways on cardiac disease pathogenesis and progression. In rodents, cardiac gene transfer has been achieved predominantly by direct injection into the myocardium or perfusion of an isolated coronary segment. Either approach results in focal overexpression of the transgene and is therefore unlikely to effectively modulate global cardiac function. Here, we provide a gene transfer method that results in global transduction of the heart. We used this technique to demonstrate in vivo that beneficial effects of SUMO1 in the setting of heart failure are dependent on SERCA2a.Further details
The protocol was published on Protocol Exchange on 13 September 2011. To see the entire protocol, click on the source link.Advertisement
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