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Non-denaturing purification of alpha-Synuclein from erythrocytes

External protocol Created on 30 Apr 2014

Authors

Tim Bartels, Joanna Choi, Nora Kim, and Dennis Selkoe

Summary

Pathogenic aggregation of α-Synuclein (αSyn) is implicated in familial and sporadic Parkinson disease (PD) and several other synucleinopathies. Choosing non-denaturing conditions, we developed a several purification schemes in order to obtain αSyn from human erythrocytes, enabling researchers to employ biophysical techniques in order to elucidate its native structure and properties.

Further details

The protocol was published on Protocol Exchange on 15 August 2011. To see the entire protocol, click on the source link.

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