Authors
Elliott Hagedorn and David Sherwood
Summary
Green fluorescent protein (GFP) and other genetically encoded fluorescent proteins provide a means to study gene expression pattern and protein localization in living tissues. Recently discovered GFP-like fluorophores and engineered variants have further expanded the fluorescent protein toolkit for in vivo imaging. Here we describe a technique using transgenic C. elegans that contain laminin or type IV collagen fused to the green-to-red photoconvertible fluorophore Dendra2, to track the fate of the basement membrane. The following protocol will allow researchers to optically highlight individual basement membrane components during developmental processes, tissue homeostasis, and aging.Further details
The protocol was published on Protocol Exchange on 16 May 2011. To see the entire protocol, click on the source link.Advertisement
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