Authors
Julie Brind'Amour and Peter M. Lansdorp
Summary
This protocol describes a method developed in our laboratory to analyze repetitive DNA in chromosomes using fluorescence in situ hybridization (FISH) and flow cytometry (chromosome flow FISH or CFF). Our protocol includes details on procedures to isolate mitotic chromosomes from adherent cell cultures, FISH using peptide nucleic acid (PNA) probes on chromosomes in suspension and flow cytometry setup and data acquisition.Further details
The protocol was published on Protocol Exchange on 4 May 2011. To see the entire protocol, click on the source link.Advertisement
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