Authors
Renzhi Han, Kazuhiro Kobuke, Mary Anderson, Daniel Beltrán-Valero de Bernabé, Yvonne Kobayashi, Baoli Yang, and Kevin Campbell
Summary
The dysferlin-null mouse line that we generated has been an invaluable tool for exploring the function of dysferlin and studying the pathogenesis of dysferlin-deficient muscular dystrophy (1-3). The complete absence of dysferlin protein in this mouse model has been demonstrated by both Western blot and immunofluorescence analyses of skeletal muscle and heart (2, 3), and these mice develop a progressive myopathy that is very similar to dysferlin-deficient muscular dystrophy in patients (3). However, the original published genotyping reaction for dysferlin-null mice can be challenging and requires two separate reactions (3). Here we report an improved genotyping protocol that is reliable and condensed to a single reaction.Further details
The protocol was published on Protocol Exchange on 27 April 2011. To see the entire protocol, click on the source link.Advertisement
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