Authors
Hanna Eilken, Michael Rieger, Philipp Hoppe, Andrea Hermann, Benjamin Smejkal, Erin Drew, Melanie Thum, Jovica Ninkovic, Ruth Beckervordersandforth, and Timm Schroeder
Summary
Long-term observation of cell behavior at the single cell level is a crucial prerequisite for understanding molecular cell fate control. This is of particular importance when analyzing highly dynamic and heterogeneous developmental and stem cell systems. The required observations are possible by time-lapse microscopy, but reliable identification of specific cell types and maturation stages usually requires detection of molecular marker expression. However, only few of the necessary cell or animal lines expressing genetically encoded fluorescent markers exist, and the generation of novel reporter lines is laborious and slow. Here, we describe long-term antibody staining in living cell cultures as an easy, robust, flexible and fast way of continuous real-time marker detection on living cells. This approach greatly simplifies the live identification of specific cell types in heterogeneous and dynamic culture systems.Further details
The protocol was published on Protocol Exchange on 28 January 2011. To see the entire protocol, click on the source link.Advertisement
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