Authors
Mirzo Kanoatov, Sahar Javaherian, and Sergey Krylov
Summary
Protein isolation from cell lysate can represent a significant challenge. The most efficient way to purify a protein is through the use of target-specific affinity ligands. Development of such affinity ligands, in most cases requires the availability of the target protein in a pure form. Thus, isolating the protein from the cell lysate requires affinity ligands, while developing affinity ligands requires a pure protein. Aptamer-facilitated Protein Isolation from Cells (AptaPIC) resolves this loop problem. AptaPIC is a technique that allows the development of aptamers for a protein in a context of a cell lysate, without any prior purification steps, and the subsequent utilization of the developed aptamers for protein isolation from the cell lysate.Further details
The protocol was published on Protocol Exchange on 13 January 2011. To see the entire protocol, click on the source link.Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 252
- Comments 0