Authors
Sriramulu Diraviam Dinesh
Summary
The artificial sputum medium (ASM) was formulated to mimic the sputum of cystic fibrosis (CF) patients. The intra- and inter-patient variation in the composition of highly viscous CF sputum complicates the achievement of a reproducible culture condition to study microbial biofilms, for example, Pseudomonas aeruginosa colonization in the CF lung. However, the ASM is a homogenous non-viscous medium, which can be prepared in bulk quantities using a fixed set of ingredients. The conventional biofilm models always had cells attached to a solid biotic or abiotic surface submerged in a steady-state or continuous flow of culture medium. In contrast, P. aeruginosa grows in the CF lung under micro-aerophilic to anaerobic conditions in the form of microcolonies eventually forming a macro-aggregate/colony in which bacteria adhere to each other and to sputum components. Similarly, in ASM, P. aeruginosa forms micro- and macro-colonies and showed various phenotypes specific to CF isolates.Further details
The protocol was published on Protocol Exchange on 24 December 2010. To see the entire protocol, click on the source link.Advertisement
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