Authors
Jodi Gureasko, William J. Galush, Holger Sondermann, Jay T. Groves, and John Kuriyan
Summary
We developed an experimental system to study the kinetics of Ras activation by the Ras activator Son-of-Sevenless (SOS) when Ras is tethered to lipid membranes. The procedure involves the covalent attachment of a C-terminal cysteine at position 181 in Ras to preformed maleimide-functionalized lipid membranes using thiol-maleimide crosslinking. Because the cysteine at position 181 in Ras undergoes posttranslational lipid modifications in vivo, the membrane anchorage of Ras at position 181 should result in the proper orientation of Ras at the membrane surface.Further details
The protocol was published on Protocol Exchange on 10 September 2010. To see the entire protocol, click on the source link.Advertisement
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