Isolation of deletion alleles of C. elegans by G4-DNA induced mutagenesis

Authors

Daphne Pontier, Evelien Kruisselbrink, Victor Guryev, and Marcel Tijsterman

Summary

Metazoan genomes contain thousands of sequence tracts that match the guanine-quadruplex (G4) DNA signature G3NxG3NxG3NxG3, a motif that is intrinsically mutagenic, probably because it can form secondary structures during DNA replication. We and others have previously shown that in C. elegans, the gene dog-1 is required to prevent deletion formation at these G4 sites (Chueng et al, Kruisselbrink et al). Here we provide a protocol to show how this feature can be used to isolate deletion alleles of many Caenorhabditis elegans genes.

Further details

The protocol was published on Protocol Exchange on 10 August 2010. To see the entire protocol, click on the source link.

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