Authors
Ting Ni, David Corcoran, Alexa Carda, Elizabeth Rach, Shen Song, Bin Xie, Yuan Gao, Uwe Ohler, and Jun Zhu
Summary
We describe an improved deep sequencing strategy, paired-end analysis of transcriptional start sites (PEAT), to globally characterize transcriptional start sites (TSSs) in a previous study [1]. It was used to explore the landscape of transcription initiation in Drosophila melanogaster embryo. Here we present a detailed protocol, with minor modifications, can be broadly applied to investigate capped transcripts in other eukaryotes, from budding yeast to rat and human cell lines.Further details
The protocol was published on Protocol Exchange on 22 July 2010. To see the entire protocol, click on the source link.Advertisement
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