Authors
Karlijn J. Wilschut Ph.D, Esther P.M. Tjin Ph.D, Henk P. Haagsman Ph.D, and Bernard A.J. Roelen Ph.D
Summary
The pig is an excellent biological model for the human supported by the anatomic similarities and the more comparable lifespan than, for instance, rodents. We explain protocols how to isolate muscle satellite cells and progenitor cells from porcine muscle tissue and to purify the side population (SP). Isolation of muscle satellite cells is based on stimulation of cell migration from muscle fibers. We describe how to purify for a specific cell population from this heterogeneous population based on Hoechst dye exclusion ability by the ABC-G2 transporter present on the SP. Furthermore, we discuss the benefits and drawbacks of each isolation method and provide lists of antibodies cross-reactive to porcine antigen epitopes for muscle stem cell characterization. Stem cells can be obtained from the pig muscle within 1 week.Further details
The protocol was published on Protocol Exchange on 2 July 2010. To see the entire protocol, click on the source link.Advertisement
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