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Preparation of yeast mitochondria and in vitro assay of respiratory chain complex activities

External protocol Created on 30 Apr 2014

Authors

Stefania Magri, Valentina Fracasso, Marco Rimoldi, and Franco Taroni

Summary

The aim of the protocol is to obtain an enriched fraction of intact mitochondria from the yeast Saccharomyces cerevisiae to perform quantitative determination of the activity of respiratory chain enzymes. All the strains used to perform this protocol are derivatives of W303 (K699: MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3-52).

Further details

The protocol was published on Protocol Exchange on 7 March 2010. To see the entire protocol, click on the source link.

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