Authors
Verena Semmling, Christoph A. Thaiss, Veronika Lukacs-Kornek, Irmgard Förster, Percy A Knolle, and Christian Kurts
Summary
Dendritic cells (DCs) attract naïve cytotoxic CD8+ T lymphocytes (CTL) by chemokines, in order to activate them for immune defense against viruses and tumors. Here we describe a protocol for quantifying such recruitment towards individual DCs in secondary lymphatics by standard immunofluorescence histology. We used reporter mice expressing green fluorescent protein under a chemokine promoter and adoptively transferred into them CTL labeled with a red fluorochrome. Tissue sections from these animals were stained with a third color identifying anatomical regions, in our case the splenic B cell zone, which encircles the T cell zone in which DCs and CTL are located. The number of CTL in that zone and their location adjacent to chemokine-producing CTL can be determined by computer-assisted digital analysis.Further details
The protocol was published on Protocol Exchange on 5 March 2010. To see the entire protocol, click on the source link.Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 290
- Comments 1