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Determination of transcription starting site of Mus musculus Melanocortin 3 Receptor gene using “New 5’RACE”

External protocol Created on 30 Apr 2014

Authors

Xin Wen, Wen Wei Zeng, and Jack A. Yanovski

Summary

TSS (Transcription starting site) determination is key to the study of transcriptional regulation, and “New 5´ RACE” (rapid amplification of cDNA ends) is a challenge method for determining multiple transcription starting sites . Since Melanocortin 3 receptor (MC3R) plays an important role in the regulation of body obesity, protocol for obtaining 5´UTR Transcription starting site of mouse Melanocortin 3 Receptor is presented.

Further details

The protocol was published on Protocol Exchange on 16 February 2010. To see the entire protocol, click on the source link.

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