Authors
Eun-Mi Ha, Kyung-Ah Lee, You Yeong Seo, Sung-Hee Kim, Jae-Hong Lim, Min-Sung Kim, Byung-Ha Oh, Jaesang Kim, and Won-Jae Lee
Summary
Unknown microbial ligand(s) other than peptidoglycan (PG) in the soluble microbial extract (SME) can induce Dual oxidase (DUOX) activity and generate reactive oxygen species in the Drosophila gut epithelia. We describe a simple method for the preparation of the PG-depleted SME (SME-ΔPG) by treatment with recombinant peptidoglycan recognition protein (PGRP)-LB, a PG-degrading enzyme. (SME-ΔPG) has been successfully used for dissecting the PG-independent immune signaling pathways in Drosophila gut. The (SME-ΔPG) will be a potentially useful material to dissect PG-independent host response to microbes in diverse biological systems.Further details
The protocol was published on Protocol Exchange on 17 August 2009. To see the entire protocol, click on the source link.Advertisement
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