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Immunostaining of Nav1.6 and Nav1.2 channels in cortical pyramidal neurons

External protocol Created on 30 Apr 2014

Authors

Cuiping Tian, Wenqin Hu, and Yousheng Shu

Summary

This protocol describes the immunohistochemical staining of Na+ channel subtypes (Nav1.2, Nav1.6) and channel-associated protein ankyrin-G (AnkG) in layer 5 pyramidal neurons of the rat neocortex. This specific staining requires a very light fixation of the brain tissue.

Further details

The protocol was published on Protocol Exchange on 29 July 2009. To see the entire protocol, click on the source link.

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