Authors
Mirjam Wamelink, Erwin Jansen, Eduard Struys, Hans Lehrach, Cornelis Jakobs, and Markus Ralser
Summary
The pentose phosphate pathway (PPP) is a central pathway of the cellular carbohydrate metabolism. The PPP is directly interconnected with glycolysis and composed of a irreversible, oxidative- and a reversible, non-oxidative part. The PPP provides the cell with intermediates for several bioorganic syntheses, and acts as balancer for the redox state in response to oxidant exposures 1,2. Here, we provide a liquid chromatography tandem mass spectrometry (LC-MS/MS) based protocol for accurate quantification of PPP intermediates in S. cerevisiae.Further details
The protocol was published on Protocol Exchange on 14 July 2009. To see the entire protocol, click on the source link.Advertisement
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