Authors
Rory Koenen and Christian Weber
Summary
Chemokines are small proteins that have an important function in the trafficking of immune cells. Platelets also contain chemokines, which are stored in the alpha-granula. Activated platelets are able to deposit chemokines onto inflamed endothelium via transient rolling interactions, thereby enhancing subsequent arrest of e.g. monocytes. The chemokines RANTES/CCL5 and platelet factor 4 (PF4/CXCL4) are deposited by platelets and can form heteromers. These CCL5/CXCL4 heteromers are particularly potent as monocyte attractant. This protocol describes the precipitation of the CCL5/CXCL4 complex from platelets.Further details
The protocol was published on Protocol Exchange on 9 June 2009. To see the entire protocol, click on the source link.Advertisement
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